Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system

@article{Livet2007TransgenicSF,
  title={Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system},
  author={Jean Livet and Tamily A. Weissman and Hyuno Kang and Ryan W. Draft and Ju Lu and Robyn A. Bennis and Joshua R. Sanes and Jeff William Lichtman},
  journal={Nature},
  year={2007},
  volume={450},
  pages={56-62}
}
Detailed analysis of neuronal network architecture requires the development of new methods. Here we present strategies to visualize synaptic circuits by genetically labelling neurons with multiple, distinct colours. In Brainbow transgenes, Cre/lox recombination is used to create a stochastic choice of expression between three or more fluorescent proteins (XFPs). Integration of tandem Brainbow copies in transgenic mice yielded combinatorial XFP expression, and thus many colours, thereby… Expand
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NEW TOOLS FOR THE BRAINBOW TOOLBOX
TLDR
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References

SHOWING 1-10 OF 51 REFERENCES
Imaging Neuronal Subsets in Transgenic Mice Expressing Multiple Spectral Variants of GFP
TLDR
Each of 25 independently generated transgenic lines expressed XFP in a unique pattern, even though all incorporated identical regulatory elements (from the thyl gene), for example, all retinal ganglion cells or many cortical neurons were XFP positive in some lines, whereas only a few ganglions or only layer 5 cortical pyramids were labeled in others. Expand
A gene expression atlas of the central nervous system based on bacterial artificial chromosomes
TLDR
A large-scale screen is described to create an atlas of CNS gene expression at the cellular level, and to provide a library of verified bacterial artificial chromosome vectors and transgenic mouse lines that offer experimental access to CNS regions, cell classes and pathways. Expand
Fluorescent Proteins Expressed in Mouse Transgenic Lines Mark Subsets of Glia, Neurons, Macrophages, and Dendritic Cells for Vital Examination
TLDR
It is shown that lines with labeled macrophages can be used to follow the accumulation of these cells at sites of injury and suggests that Schwann cells at individual synapses are clonally related, a finding with implications for how these cells might be sorted during postnatal development. Expand
Overexpression of growth-associated proteins in the neurons of adult transgenic mice
  • P. Caroni
  • Medicine, Biology
  • Journal of Neuroscience Methods
  • 1997
TLDR
The Thy-1.2 expression cassette described in this report drives strong constitutive expression of transgenes specifically in the neurons of adult transgenic mice, with the added opportunity of analyzing different combinations of expressing and non-expressing neurons in transgenic animals derived from different founder lines. Expand
Mosaic Analysis with Double Markers in Mice
TLDR
Cerebellar granule cell progenitors are fated at an early stage to produce granule cells with axonal projections limited to specific sublayers of the cerebellar cortex, showing that interchromosomal recombination can be induced efficiently in vivo in both mitotic and postmitotic cells in all tissues examined. Expand
Genome-wide atlas of gene expression in the adult mouse brain
TLDR
An anatomically comprehensive digital atlas containing the expression patterns of ∼20,000 genes in the adult mouse brain is described, providing an open, primary data resource for a wide variety of further studies concerning brain organization and function. Expand
Cortical Excitatory Neurons and Glia, But Not GABAergic Neurons, Are Produced in the Emx1-Expressing Lineage
TLDR
A strain of mice is generated that expresses the Cre recombinase in a spatial and temporal pattern like that observed for Emx1, and it is demonstrated that radial glia, Cajal-Retzius cells, glutamatergic neurons, astrocytes, and oligodendrocytic cells of most pallial structures originate from an EmX1-expressing lineage. Expand
Multicolor “DiOlistic” Labeling of the Nervous System Using Lipophilic Dye Combinations
TLDR
The Golgi-like labeling of neurons with many different colors provides a novel way to study neuronal connectivity and is useful for studying structural plasticity of neuronal circuits in living preparations. Expand
Transient requirement for ganglion cells during assembly of retinal synaptic layers
TLDR
The lak mutant phenotype reveals that ganglion cells have a transient role organizing the earliest amacrine projections to the IPL, and suggest that amacrines may guide IPL sublamination by providing stratification cues for other cell types. Expand
Genetic analysis of the homeodomain transcription factor Chx10 in the retina using a novel multifunctional BAC transgenic mouse reporter.
TLDR
Crosses of the Chx10 BAC reporter mice to R26R mice for fate-mapping experiments revealed that Chx 10 reporter-expressing progenitor cells contribute to all mature cell types of the retina. Expand
...
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2
3
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