Transgenic silkworms (Bombyx mori) produce recombinant spider dragline silk in cocoons

@article{Wen2009TransgenicS,
  title={Transgenic silkworms (Bombyx mori) produce recombinant spider dragline silk in cocoons},
  author={Hongxiu Wen and Xiqian Lan and Yuansong Zhang and Tianfu Zhao and Yujun Wang and Zenta Kajiura and Masao Nakagaki},
  journal={Molecular Biology Reports},
  year={2009},
  volume={37},
  pages={1815-1821}
}
Spider dragline silk is a unique fibrous protein with a combination of tensile strength and elasticity, but the isolation of large amounts of silk from spiders is not feasible. In this study, we generated germline-transgenic silkworms (Bombyx mori) that spun cocoons containing recombinant spider silk. A piggyBac-based transformation vector was constructed that carried spider dragline silk (MaSp1) cDNA driven by the sericin 1 promoter. Silkworm eggs were injected with the vector, producing… 
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105 Citations

CRISPR/Cas9 Initiated Transgenic Silkworms as a Natural Spinner of Spider Silk.
TLDR
A CRISPR/Cas9 initiated fixed-point strategy was used to successfully incorporate spider silk protein genes into the Bombyx mori genome and the resulting fibers are as strong as native spider silks (1.2 GPa tensile strength).
High-Toughness Silk Produced by a Transgenic Silkworm Expressing Spider (Araneus ventricosus) Dragline Silk Protein
TLDR
To demonstrate the commercial feasibility for machine reeling, weaving, and sewing, the transgenic spider silk was used to weave a vest and scarf; this was the first application of spider silk fibers from transgenic silkworms.
Silkworms transformed with chimeric silkworm/spider silk genes spin composite silk fibers with improved mechanical properties
TLDR
These results demonstrate that silkworms can be engineered to manufacture composite silk fibers containing stably integrated spider silk protein sequences, which significantly improve the overall mechanical properties of the parental silkworm silk fibers.
Stably Express Spider Flagelliform Silk Protein in Bombyx Mori Cell Line by PiggyBac Transposon-Derived Vector
TLDR
The present results suggested that transgenic silkworm directly secreting functional spider silk protein in cocoon by using piggyBac system is feasible.
The advances and perspectives of recombinant protein production in the silk gland of silkworm Bombyx mori
  • Hanfu Xu
  • Biology, Medicine
    Transgenic Research
  • 2014
TLDR
This review recapitulates the progress of producing recombinant proteins and silk-based biomaterials in the silk gland of silkworm in addition to the construction of expression systems.
Genome engineering and parthenocloning in the silkworm, Bombyx mori
TLDR
Rational exploitation of available parthenozygous pools and the use of parthenocloning methods enable rapid fixation and maintenance of the desired genotypes and conserve genetic traits and improve selection and amplification of clones containing genes of interest.
Advanced silk material spun by a transgenic silkworm promotes cell proliferation for biomedical application.
Genome editing of BmFib-H gene provides an empty Bombyx mori silk gland for a highly efficient bioreactor
TLDR
It is shown that removal of endogenous Bmfib-H protein could significantly increase the expression level of exogenous protein, and it is demonstrated that the bioreactor is suitable for large scale production of protein-based materials.
Genome engineering and parthenocloning in the silkworm,Bombyxmori
TLDR
Rational exploitation of available parthenozygous pools and the use of parthenocloning methods enable rapid fixation and maintenance of the desired genotypes and conserve genetic traits and improve selection and amplification of clones containing genes of interest.
Recombinant production of spider silk proteins.
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