Transgenic plants of Agrostis alba obtained by electroporation-mediated direct gene transfer into protoplasts

@article{Asano1994TransgenicPO,
  title={Transgenic plants of Agrostis alba obtained by electroporation-mediated direct gene transfer into protoplasts},
  author={Yoshito Asano and Masashi Ugaki},
  journal={Plant Cell Reports},
  year={1994},
  volume={13},
  pages={243-246}
}
A system for genetic transformation of Agrostis alba plants by electroporation-mediated DNA transfer to protoplasts is described. The npt II gene was used as a selectable marker. Selection with 20 mg/1 G418 (geneticin) yielded a total of over 50 resistant cell colonies from three independent experiments. Overall frequency of resistant colony formation was 1–3 × 10−6 based on the number of protoplasts plated and 1–2 × 10−5 based on the number of cell colonies recovered. Subsequent subcultures… CONTINUE READING
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