Transgenic plant virus resistance mediated by untranslatable sense RNAs: expression, regulation, and fate of nonessential RNAs.

@article{Smith1994TransgenicPV,
  title={Transgenic plant virus resistance mediated by untranslatable sense RNAs: expression, regulation, and fate of nonessential RNAs.},
  author={Heath A Smith and S L Swaney and T. Dawn Parks and Earl A. Wernsman and W. G. Dougherty},
  journal={The Plant cell},
  year={1994},
  volume={6 10},
  pages={1441-53}
}
Haploid leaf tissue of tobacco cultivars K326 and K149 was transformed with several transgenes containing cDNA of the potato virus Y (PVY) coat protein (CP) open reading frame (ORF). The various transgenes containing the PVY CP ORF sequence produced (1) the expected mRNA and CP product, (2) an mRNA rendered untranslatable by introduction of a stop codon immediately after the initiation codon, or (3) an antisense RNA that was untranslatable as a result of the incorrect orientation of the PVY CP… CONTINUE READING
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