Transgenic mice overexpressing tartrate-resistant acid phosphatase exhibit an increased rate of bone turnover.

@article{Angel2000TransgenicMO,
  title={Transgenic mice overexpressing tartrate-resistant acid phosphatase exhibit an increased rate of bone turnover.},
  author={Nicola Z. Angel and Nicole Walsh and Mark R. Forwood and Michael C. Ostrowski and Alan I. Cassady and David A. Hume},
  journal={Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research},
  year={2000},
  volume={15 1},
  pages={103-10}
}
Tartrate-resistant acid phosphatase (TRAP) is a secreted product of osteoclasts and a lysosomal hydrolase of some tissue macrophages. To determine whether TRAP expression is rate-limiting in bone resorption, we overexpressed TRAP in transgenic mice by introducing additional copies of the TRAP gene that contained the SV40 enhancer. In multiple independent mouse lines, the transgene gave a copy number-dependent increase in TRAP mRNA levels and TRAP activity in osteoclasts, macrophages, serum, and… CONTINUE READING