Transgenic mice expressing a human poliovirus receptor: A new model for poliomyelitis

@article{Ren1990TransgenicME,
  title={Transgenic mice expressing a human poliovirus receptor: A new model for poliomyelitis},
  author={Ruibao Ren and Frank D Costantini and EDWARD J. Gorgacz and James J. Lee and Vincent R. Racaniello},
  journal={Cell},
  year={1990},
  volume={63},
  pages={353-362}
}
A human poliovirus receptor (PVR) gene was used to generate transgenic mice that express PVR transcripts and poliovirus binding sites in a wide range of tissues. Intracerebral inoculation of PVR transgenic mice with poliovirus type 1, Mahoney strain, resulted in viral replication in the brain and spinal cord and development of paralytic poliomyelitis. P1/Mahoney did not replicate or cause paralysis in nontransgenic mice. PVR transgenic mice failed to develop clinical disease when inoculated… 
A Transgenic Mouse Model of Poliomyelitis.
TLDR
Assessment of the neurovirulence levels of poliovirus strains, including mutant viruses produced by reverse genetics, circulating vaccine-derivedPoliovirus, and vaccine candidates, is useful for basic research of pol Giovirus pathogenicity, the surveillance of circulating polioviruses, and the quality control of oral live poliov virus vaccines, and does not require the use of monkeys.
Transgenic mice and the pathogenesis of poliomyelitis.
TLDR
Despite the widespread pattern of PVR expression, poliov virus infection in TgPVR mice is restricted to only a few sites, indicating that poliovirus tropism is not controlled solely by the ability of cells to bind virus.
Transgenic mice carrying the human poliovirus receptor: new animal models for study of poliovirus neurovirulence
TLDR
The results of this study suggest that the Tg mice used in this study may be suitable for replacing monkeys for investigating poliovirus neurovirulence and values of 50% lethal dose are useful to score a wide range of neurovirulent viruses.
Comparison of neuropathogenicity of poliovirus in two transgenic mouse strains expressing human poliovirus receptor with different distribution patterns.
TLDR
The results suggest that the neuropathogenicity of poliovirus changes markedly depending on the specific expression of the PVR molecule in the central nervous system.
Poliovirus pathogenesis in a new poliovirus receptor transgenic mouse model: age-dependent paralysis and a mucosal route of infection.
TLDR
The PVR transgenic mouse reported here provides the first available model for a mucosal route of infection with poliovirus, suggesting that intranasal infection of cPVR mice is a model for bulbar paralysis.
Human poliovirus receptor gene expression and poliovirus tissue tropism in transgenic mice
TLDR
It is demonstrated that poliovirus tissue tropism is not governed solely by expression of the PVR gene nor by accessibility of cells to virus.
The Alpha/Beta Interferon Response Controls Tissue Tropism and Pathogenicity of Poliovirus
TLDR
It is considered that poliovirus replication was observed in the nontarget tissues of PVR-transgenic/Ifnar knockout mice because the IFN response was null in all tissues.
Expression of the poliovirus receptor in intestinal epithelial cells is not sufficient to permit poliovirus replication in the mouse gut
TLDR
The results indicate that the inability of poliovirus to replicate in the mouse alimentary tract is not solely due to the absence of virus receptor, and other factors are involved in determining the ability of poliomyelitis-related infection by the oral route.
Poliomyelitis in intraspinally inoculated poliovirus receptor transgenic mice.
TLDR
CNS inoculations with type III polioviruses differing in relative neurovirulence show that these mouse lines are similar in disease frequency and severity, demonstrating that differences in receptor gene dosage and concomitant receptor abundance do not affect susceptibility to infection.
Establishment of a Poliovirus Oral Infection System in Human Poliovirus Receptor-Expressing Transgenic Mice That Are Deficient in Alpha/Beta Interferon Receptor
TLDR
It is shown that PV is inactivated by the low pH of the gastric contents in mice, and IFNAR plays an important role in determining permissivity in the alimentary tract as well as the generation of virus-specific immune responses to PV via the oral route.
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