Transgenic expression of green fluorescent protein in caprine embryos produced through electroporation-aided sperm-mediated gene transfer.

@article{Pramod2016TransgenicEO,
  title={Transgenic expression of green fluorescent protein in caprine embryos produced through electroporation-aided sperm-mediated gene transfer.},
  author={R Kumar Pramod and Rakesh Kumar and Abhijit Mitra},
  journal={Gene},
  year={2016},
  volume={576 1 Pt 3},
  pages={505-11}
}
Current methods of transgenic animal production are afflicted by low efficiency and high cost. Recently, the electroporation aided sperm-mediated gene transfer (SMGT) emerges as a promising alternative with variable success rate. Among the domestic animal species, the electroporation-aided SMGT is less investigated in goats, except a few reports in which attempts have been made using the auto-uptake method of SMGT. In this study, we report an optimized electroporation condition for SMGT of… CONTINUE READING