Transgenic Tobacco Plants Expressing a Dimeric Single-chain Variable Fragment (scFv) Antibody Against Salmonella enterica Serotype Paratyphi B

@article{MakvandiNejad2005TransgenicTP,
  title={Transgenic Tobacco Plants Expressing a Dimeric Single-chain Variable Fragment (scFv) Antibody Against Salmonella enterica Serotype Paratyphi B},
  author={Shokouh Makvandi-Nejad and Michael D. McLean and Tomoko Hirama and Kurt C. Almquist and Colin R MacKenzie and J. Christopher Hall},
  journal={Transgenic Research},
  year={2005},
  volume={14},
  pages={785-792}
}
Transgenic tobacco plants were produced that express an anti-Salmonella enterica single-chain variable fragment (scFv) antibody that binds to the lipopolysaccharide (LPS) of S. enterica Paratyphi B. The coding sequence of this scFv was optimized for expression in tobacco, synthesized and subsequently placed behind three different promoters: an enhanced tobacco constitutive ubiquitous promoter (EntCUP4), and single- and double-enhancer versions of the Cauliflower Mosaic Virus 35S promoter (CaMV… CONTINUE READING

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