Transgenesis using nuclear transfer in goats.

  title={Transgenesis using nuclear transfer in goats.},
  author={Anthoula Lazaris and Rebecca Keyston and Costas N. Karatzas and Carol Keefer},
  journal={Methods in molecular biology},
Nuclear transfer (NT) using transgenic donor cells is an efficient means for generation of transgenic founder goats, especially in regard to the number of animals required to produce a transgenic founder expressing the protein of interest. Vectors can be designed for organ-specific expression and secretion of recombinant proteins within the target tissue. Furthermore, donor cells can be selected for gender, genetically modified to introduce the transgene of interest and screened for… CONTINUE READING

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