Transgene integration in aspen: structures of integration sites and mechanism of T-DNA integration.

@article{Kumar2002TransgeneII,
  title={Transgene integration in aspen: structures of integration sites and mechanism of T-DNA integration.},
  author={Sandeep Kumar and Matthias Fladung},
  journal={The Plant journal : for cell and molecular biology},
  year={2002},
  volume={31 4},
  pages={543-51}
}
To obtain insight into the mechanism of transferred DNA (T-DNA) integration in a long-lived tree system, we analysed 30 transgenic aspen lines. In total, 27 right T-DNA/plant junctions, 20 left T-DNA/plant junctions, and 10 target insertions from control plants were obtained. At the right end, the T-DNA was conserved up to the cleavage site in 18 transgenic lines (67%), and the right border repeat was deleted in nine junctions. Nucleotides from the left border repeat were present in 19… CONTINUE READING

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