Transgene-free genome editing in Caenorhabditis elegans using CRISPR-Cas.

@article{Chiu2013TransgenefreeGE,
  title={Transgene-free genome editing in Caenorhabditis elegans using CRISPR-Cas.},
  author={Hui Wen Chiu and Hillel Tsvi Schwartz and Igor Antoshechkin and Paul W. Sternberg},
  journal={Genetics},
  year={2013},
  volume={195 3},
  pages={
          1167-71
        }
}
CRISPR-Cas is an efficient method for genome editing in organisms from bacteria to human cells. We describe a transgene-free method for CRISPR-Cas-mediated cleavage in nematodes, enabling RNA-homology-targeted deletions that cause loss of gene function; analysis of whole-genome sequencing indicates that the nuclease activity is highly specific. 

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References

Publications referenced by this paper.
SHOWING 1-7 OF 7 REFERENCES

Improved detection of small deletions in complex pools of DNA

Y. B. Tzur, K. M. Esvelt, M. P. Colaiacovo, G. M. Church
  • Nucleic Acids Res .
  • 2013
VIEW 1 EXCERPT

TheMillion Mutation Project : a new approach to genetics in Caenorhabditis elegans

A. E. Friedland, S. Nadarajan, G. M. Church, J. A. Calarco
  • Genome Res .
  • 2013
VIEW 2 EXCERPTS

Engineering the Caenorhabditis elegans genome using the CRISPR - Cas 9 system and homologous recombination

A. D’Souza, G. Moulder, S. McKay, B. Shen