Transgene expression from the Tribolium castaneum Polyubiquitin promoter

@article{Lorenzen2002TransgeneEF,
  title={Transgene expression from the Tribolium castaneum Polyubiquitin promoter},
  author={Marc{\'e} D. Lorenzen and S J Brown and Robin E. Denell and Richard W. Beeman},
  journal={Insect Molecular Biology},
  year={2002},
  volume={11}
}
The highly conserved Ubiquitin proteins are expressed from genes with strong, constitutively active promoters in many species, making these promoters attractive candidates for use in driving transgene expression. Here we report the cloning and characterization of the Tribolium castaneum Polyubiquitin (TcPUb) gene. We placed the TcPUb promoter upstream of the coding region of the T. castaneum eye‐colour gene Tc vermilion (Tcv) and injected this construct into embryos from a Tcv‐deficient strain… Expand
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