Transgene-coded chimeric proteins as reporters of intracellular proteolysis: starvation-induced catabolism of a lacZ fusion protein in muscle cells of Caenorhabditis elegans.

@article{Zdinak1997TransgenecodedCP,
  title={Transgene-coded chimeric proteins as reporters of intracellular proteolysis: starvation-induced catabolism of a lacZ fusion protein in muscle cells of Caenorhabditis elegans.},
  author={L A Zdinak and Ira B. Greenberg and Nathaniel J Szewczyk and Sami J Barmada and M Cardamone-Rayner and James J Hartman and Lewis A. Jacobson},
  journal={Journal of cellular biochemistry},
  year={1997},
  volume={67 1},
  pages={143-53}
}
The product of an integrated transgene provides a convenient and cell-specific reporter of intracellular protein catabolism in 103 muscle cells of the nematode Caenorhabditis elegans. The transgene is an in-frame fusion of a 5'-region of the C. elegans unc-54 (muscle myosin heavy-chain) gene to the lacZ gene of Escherichia coli [Fire and Waterston (1989): EMBO J 8:3419-3428], encoding a 146-kDa fusion polypeptide that forms active beta-galactosidase tetramers. The protein is stable in vivo in… CONTINUE READING

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