Transforming growth factor type beta: rapid induction of fibrosis and angiogenesis in vivo and stimulation of collagen formation in vitro.

  title={Transforming growth factor type beta: rapid induction of fibrosis and angiogenesis in vivo and stimulation of collagen formation in vitro.},
  author={A. Roberts and M. Sporn and R. Assoian and J. M. Smith and N. Roche and L. Wakefield and U. Heine and L. Liotta and V. Falanga and J. Kehrl},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={83 12},
  • A. Roberts, M. Sporn, +7 authors J. Kehrl
  • Published 1986
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
Transforming growth factor type beta (TGF-beta), when injected subcutaneously in newborn mice, causes formation of granulation tissue (induction of angiogenesis and activation of fibroblasts to produce collagen) at the site of injection. These effects occur within 2-3 days at dose levels than 1 microgram. Parallel in vitro studies show that TGF-beta causes marked increase of either proline or leucine incorporation into collagen in either an NRK rat fibroblast cell line or early passage human… Expand
Stimulation of fibroblast cell growth, matrix production, and granulation tissue formation by connective tissue growth factor.
It is demonstrated that, like TGF-beta, CTGF can induce connective tissue cell proliferation and extracellular matrix synthesis. Expand
Transforming growth factor-beta stimulates collagen and fibronectin synthesis by human corneal stromal fibroblasts in vitro.
Both TGF beta and EGF are potent stimulants of collagen and fibronectin synthesis and proliferation and therefore, these two growth factors may be effective alternatives or additional choices for the treatment of corneal ulcer. Expand
Platelet-derived growth factor and transforming growth factor-beta enhance tissue repair activities by unique mechanisms
In contrast, PDGF is a more potent chemoattractant for wound macrophages and fibroblasts and may stimulate these cells to express endogenous growth factors, including TGF-beta, which may stimulate new collagen synthesis and sustained enhancement of wound healing over a more prolonged period of time. Expand
Effect of growth factors on collagen metabolism in cultured human heart fibroblasts.
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Multiple regulatory effects by transforming growth factor-beta on type I collagen levels in osteoblast-enriched cultures from fetal rat bone.
TGF beta 1 has multiple effects on type I collagen in fetal bone-derived cell cultures, including small increases in mRNA, large increases in polypeptide synthesis, and enhanced association of secreted collagen to the cell layer, which may require synthesis of extracellular components unrelated to fibronectin or the beta 1-integrin subunit. Expand
Collagen matrices attenuate the collagen-synthetic response of cultured fibroblasts to TGF-beta.
Collagen matrices attenuate the collagen synthetic response of fibroblast to transforming growth factor-beta in vitro and possibly in vivo, resulting in reduced collagen synthesis in fibroblasts cultured on plastic. Expand
Transforming growth factor beta (TGF beta) causes a persistent increase in steady-state amounts of type I and type III collagen and fibronectin mRNAs in normal human dermal fibroblasts.
The results indicate that TGF beta causes a marked enhancement of the production of types I and III collagens and fibronectin by cultured normal human dermal fibroblasts and may contribute to the development of pathological states of fibrosis. Expand
Stimulatory effects of transforming growth factor-beta and epidermal growth factor on epidermal cell outgrowth from porcine skin explant cultures.
  • P. Hebda
  • Biology, Medicine
  • The Journal of investigative dermatology
  • 1988
The results indicate that TGF-beta produced earlier initiation of outgrowth, by 1-2 d compared with control cultures, and increased the rate of out growth during the migratory phase of culture, and suggest that T GF-beta may play a role in early epidermal wound healing. Expand
Modulation of collagen gene expression by cytokines: stimulatory effect of transforming growth factor-beta1, with divergent effects of epidermal growth factor and tumor necrosis factor-alpha on collagen type I and collagen type IV.
Observations support a dominant role for TGF-beta1 in stimulating coordinate expression of collagen type I and collagen type IV mRNAs by NIH-3T3 cells; EGF and TNF-alpha are capable of inducing divergent expression of the genes for these two types of collagen. Expand
Stimulation of the chemotactic migration of human fibroblasts by transforming growth factor beta
Since TGF-beta is secreted by a variety of neoplastic and normal cells including platelets, monocytes/macrophages, and lymphocytes, it may play a critical role in vivo in embryogenesis, host response to tumors, and the repair response that follows damage to tissues by immune and nonimmune reactions. Expand


Human transforming growth factor-α causes precocious eyelid opening in newborn mice
It is demonstrated that human TGF-α is as active as murine EGF in promoting eyelid opening in newborn mice and in a dose-dependent eyelidOpening assay that human EGF is as potent as its murine homologue with respect to this biological property. Expand
Pathologic basis of disease
The objective is to establish an experimental procedure and show direct AFM progression from EMT to EMT using a simple, straightforward, and reproducible procedure. Expand