Transforming a (beta/alpha)8--barrel enzyme into a split-protein sensor through directed evolution.

Abstract

Split-protein sensors have become an important tool for the analysis of protein-protein interactions in living cells. We present here a combinatorial method for the generation of new split-protein sensors and demonstrate its application toward the (beta/alpha)(8)-barrel enzyme N-(5'-phosphoribosyl)-anthranilate isomerase Trp1p from Saccharomyces cerevisiae… (More)

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Cite this paper

@article{Tafelmeyer2004TransformingA, title={Transforming a (beta/alpha)8--barrel enzyme into a split-protein sensor through directed evolution.}, author={Petra Tafelmeyer and Nils Johnsson and Kai Johnsson}, journal={Chemistry & biology}, year={2004}, volume={11 5}, pages={681-9} }