Transformation ofBacillus subtilis with the treatment by alkali cations

Abstract

ExposingBacillus subtilis cultures to high concentrations of alkali cations, especially K+, allows efficient transformation by plasmids. The method allows transformation with unfractionated plasmid DNA, monomeric plasmid DNA as well as linear plasmid DNA.B. subtilis strains, not amenable to natural transformation, were also transformed by the present method.

DOI: 10.1007/BF01022423

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Cite this paper

@article{Ano2005TransformationOS, title={Transformation ofBacillus subtilis with the treatment by alkali cations}, author={Takashi Ano and Akira Kobayashi and Makoto Shoda}, journal={Biotechnology Letters}, year={2005}, volume={12}, pages={99-104} }