Transformation of white poplar (Populus alba L.) with a novel Arabidopsis thaliana cysteine proteinase inhibitor and analysis of insect pest resistance

  title={Transformation of white poplar (Populus alba L.) with a novel Arabidopsis thaliana cysteine proteinase inhibitor and analysis of insect pest resistance},
  author={Massimo Delledonne and Gianni Allegro and Beatrice Belenghi and Alma Balestrazzi and Franco Picco and Alex Levine and Samantha Zelasco and Paolo Calligari and Massimo Confalonieri},
  journal={Molecular Breeding},
Transgenic white poplar (Populus alba L.) plants expressing a novel Arabidopsis thaliana cysteine proteinase inhibitor (Atcys) gene have been produced using Agrobacterium tumefaciens-mediated gene transfer. Internodal stem segments of cv. Villafranca were co-cultivated with the EHA105 pBI-Atcys A. tumefaciens strain. Sixteen putative transgenic plant lines were regenerated from different calli with a transformation efficiency of 11%. The integration and expression of the cysteine proteinase… CONTINUE READING
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A revised medium for rapid growth and bioassays with tobacco tissue cultures

  • T. Murashige, F. Skoog
  • Physiol. Plant. 15: 73–97.
  • 1962
Highly Influential
5 Excerpts

Toxicity to Chrysomela tremulae(Coleoptera: Chrysomelidae) of transgenic poplars expressing a cysteine proteinase inhibitor

  • J. C. Leplé, M. Bonadé Bottino, +5 authors L. Jouanin
  • Mol. Breed. 1: 319–328.
  • 1995
Highly Influential
4 Excerpts

Commercially feasible micropropagation of mountain laurel

  • G. Lloyd, B. H. McCown
  • 1991
Highly Influential
4 Excerpts

Proteinase inhibitor in plants: genes for improving defenses against insects and pathogens

  • C. A. Ryan
  • Annu. Rev. Phytopath. 28: 425–449.
  • 1990
Highly Influential
3 Excerpts

Molecular Cloning: A Laboratory manual, 2nd ed

  • J. Sambrook, E. F. Fritsch, T. Maniatis
  • Cold Spring Harbor Laboratory Press, Cold Spring…
  • 1989
Highly Influential
2 Excerpts

La difesa del vivaio di pioppo

  • G. Allegro, G. Giorcelli
  • Inform. Agrario 47(26): 49–56.
  • 1997
1 Excerpt

Micropropagation, Genetic Engineering, and Molecular Biology of Populus

  • N. B. Klopfenstein, Y. W. Chun, M. S. Kim, M. R. Ahuja
  • General Technical Report RM-GTR-297. USDA Fort…
  • 1997
2 Excerpts

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