Transformation of various species of gram-negative bacteria belonging to 11 different genera by electroporation

@article{Wirth2004TransformationOV,
  title={Transformation of various species of gram-negative bacteria belonging to 11 different genera by electroporation},
  author={Reinhard Wirth and Anita Friesenegger and Stefan Fiedler},
  journal={Molecular and General Genetics MGG},
  year={2004},
  volume={216},
  pages={175-177}
}
SummaryWe have undertaken a systematic study to test the transformation of various species of gram-negative bacteria using the electroporation method. The data obtained show very clearly that a great variety of gram-negative bacteria — 15 different species belonging to 11 different genera — including freshly isolated wild-type strains can be transformed efficiently by use of the electric-field mediated transformation technique. These include species of the families Enterobacteriaceae… Expand
Methodologies to increase the transformation efficiencies and the range of bacteria that can be transformed
TLDR
This review presents the progress on the development of methods for artificial transformation of bacteria with emphasis on different methodologies and the range of bacteria that can be transformed. Expand
Electroporation of Bradyrhizobium japonicum
TLDR
Electroporation offers a fast, efficient and reproducible way to introduce DNA into bacteria and routine isolation of DNA from an E. coli strain incapable of DNA modification should help in increasing transformation efficiencies for other strains of bacteria where DNA restriction appears to be a significant obstacle to successful transformation. Expand
Development of a P 1 phagemid system for the delivery of DNA into Gram-negative bacteria
Department of Microbiology and Immunology, Medical University of South Carolina, 173 Ashley Avenue BSB-201, Charleston, SC 29403, USA The inability to transform many clinically importantExpand
Development of a P1 phagemid system for the delivery of DNA into Gram-negative bacteria.
TLDR
This report describes the development and construction of a delivery system utilizing the broad-host-range transducing bacteriophage P1 that was used successfully to deliver phagemids to a number of strains isolated from patients. Expand
Transformation of Acinetobacter baumannii: Electroporation.
TLDR
An optimized electroporation protocol and guidance for electroporers of clinical MDR isolates of A. baumannii and its essential genes is provided. Expand
High efficiency transformation of Salmonella typhimurium and Salmonella typhi by electroporation
TLDR
Under these conditions electroporation can be used for the routine and direct transformation of Salmonella strains with partially purified (alkaline lysis) plasmid DNA from E. coli. Expand
High-Frequency Lithium Acetate Transformation of Schizosaccharomyces pombe.
TLDR
An efficient transformation procedure for use in the fission yeast Schizosaccharomyces pombe, which relies on chemical agents and temperature stresses to facilitate transfer of the genetic material through the cell wall and plasma membrane without significant impact on the transferred DNA or the recipient cell. Expand
Improvement of electroporation-mediated transformation efficiency for a Bifidobacterium strain to a reproducibly high level.
TLDR
The optimized electrotransformation conditions reported here should be widely applicable to other Bifidobacterium species, these could promote the extensive genetic manipulation of the various B ifidobacteria species in future studies. Expand
Artificial transformation methodologies for improving the efficiency of plasmid DNA transformation and simplifying its use
TLDR
In this review, artificial transformation methods have been classified according to the membrane-permeabilizing mechanisms employed by them according to their influential factors, transformation efficiency, advantages, disadvantages, and practical applications. Expand
Tools for genetic manipulation of the plant growth-promoting bacterium Azospirillum amazonense
TLDR
A promoter analysis protocol based on fluorescent protein expression was optimized to aid genetic regulation studies on this bacterium, and could provide a better understanding of the genetic mechanisms of this species that underlie its plant growth promotion. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 14 REFERENCES
Transformation of bacteria with plasmid DNA by electroporation.
TLDR
The possibility of electric field-mediated transformation ("electroporation") of a gram-positive bacterium and two gram-negative bacteria with plasmid DNA was investigated and results are given. Expand
Introduction of plasmid DNA into Streptomyces lividans by electroporation
A new procedure for introducing plasmid DNA into Streptomyces lividans protoplasts by electroporation is described. Transformation of S. lividans by plasmid DNA was observed under various conditionsExpand
Transformation of Lactobacillus casei by electroporation
TLDR
Current methods for the transfection and transformation of lactobacilli using protoplasts are slow, inefficient, and inconsistent, and electroporation was evaluated as a simple alternative. Expand
Highly efficient protoplast transformation system for Streptococcus faecalis and a new Escherichia coli-S. faecalis shuttle vector
TLDR
A shotgun cloning experiment ligated a tetracycline resistance determinant from Streptococcus sanguis chromosomal DNA into pAM401 by direct transformation of S. faecalis, establishing the utility of the protoplast transformation system and of the new shuttle vector. Expand
High efficiency transformation of E. coli by high voltage electroporation
TLDR
E. coli can be transformed to extremely high efficiencies by subjecting a mixture of cells and DNA to brief but intense electrical fields of exponential decay waveform (electroporation), and most of the surviving cells are competent with up to 80% transformed at high DNA concentration. Expand
Transformation of Streptococcus lactis Protoplasts by Plasmid DNA
TLDR
Polyethylene glycol-treated protoplasts prepared from Streptococcus lactis LM3302 were transformed to lactose-fermenting ability by a transductionally shortened plasmid (pLM2103) coding for lactose utilization. Expand
Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas.
TLDR
A cosmid that may be used for the selective cloning of large DNA fragments by in vitro lambda packaging and an analogous series of vectors that are defective in their plasmid-mobilization function, and that exhibit a degree of biological containment comparable to that of current Escherichia coli vector plasmids, are described. Expand
Molecular Cloning: A Laboratory Manual
TLDR
The content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Expand
A Laboratory manual
TLDR
To develop a program to print the barcodes using two commonly uses command sets and hence evaluates their ease of use for such applications, students should be able to program dot matrix printers, by manipulating bit level information and ink jet printers using page description language, such as PCL. Expand
Transformation of bacteria with plasmid
  • 1988
...
1
2
...