Transformation of the oilseed crop Camelina sativa by Agrobacterium-mediated floral dip and simple large-scale screening of transformants

@article{Liu2012TransformationOT,
  title={Transformation of the oilseed crop Camelina sativa by Agrobacterium-mediated floral dip and simple large-scale screening of transformants},
  author={Xunjia Liu and Jennifer M. Brost and Carolyn Hutcheon and Robin Guilfoil and Anna Wilson and Sharon Tsui-Shan Leung and Christine K. Shewmaker and Suzanne Rooke and Thu A Nguyen and Jack Kiser and Jay De Rocher},
  journal={In Vitro Cellular & Developmental Biology - Plant},
  year={2012},
  volume={48},
  pages={462-468}
}
Camelina sativa is a promising under-exploited oilseed crop with potential to become a biofuel feedstock. The ability to transform C. sativa would allow for the rapid introduction of novel traits into this emerging crop. We report the development of an Agrobacterium-based floral dip transformation method, requiring no vacuum-infiltration step, with transformation efficiencies up to 0.8%. C. sativa cultivars Ames 26665, “Calena” A3U7761, Ames 1043, and “Celine” were tested using Agrobacterium… CONTINUE READING
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