Transformation of the moss Physcomitrella patens using direct DNA uptake by protoplasts.

@article{Cove2009TransformationOT,
  title={Transformation of the moss Physcomitrella patens using direct DNA uptake by protoplasts.},
  author={David J. Cove and Pierre-François Perroud and Audra J. Charron and Stuart F Mcdaniel and Abha Khandelwal and Ralph S. Quatrano},
  journal={Cold Spring Harbor protocols},
  year={2009},
  volume={2009 2},
  pages={pdb.prot5143}
}
This protocol describes how to transform moss (Physcomitrella patens) protoplasts using polyethylene glycol (PEG)-mediated DNA uptake. The transformation rates for direct uptake by protoplasts of DNA with and without genomic sequence (a targeting construct) are typically 10 and 10, respectively. (These are the frequencies of stable transformants among regenerants surviving the transformation procedure.)