Transformation of psi- Saccharomyces cerevisiae to psi+ with DNA co-purified with 3 micron circles.

@article{Dai1986TransformationOP,
  title={Transformation of psi- Saccharomyces cerevisiae to psi+ with DNA co-purified with 3 micron circles.},
  author={Huacheng Dai and Selena X H Tsay and Patricia M. Lund and Brian Cox},
  journal={Current genetics},
  year={1986},
  volume={11 1},
  pages={79-82}
}
DNA enriched for supercoiled plasmids prepared from the 3 micron plasmid-enriched, [psi+], [2 micron degrees] strain 6-1G-P188 and from the [2 micron+] [psi+] strain LL20 can be used to transform a psi- recipient strain to psi+. Fractionation of the former preparation by electrophoresis showed that the 3 micron plasmid band contained the transforming activity. 

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