Transformation of plasmid DNA into Streptomyces at high frequency

@article{Bibb1978TransformationOP,
  title={Transformation of plasmid DNA into Streptomyces at high frequency},
  author={Mervyn J. Bibb and Judith M. Ward and David. Hopwood},
  journal={Nature},
  year={1978},
  volume={274},
  pages={398-400}
}
OVER 60% of known antibiotics1 are produced by Streptomyces species, including many substances with valuable clinical and other applications; they therefore have considerable medical, biological and commercial importance. Although the process of gene exchange mediated by conjugation within these actinomycetes is apparently widespread2, the transfer of genetic material between individuals by such sexual means is, by definition, predominantly restricted to members of the same species. Transfer of… 
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Progress is described in the program to apply recombinant DNA technology to S. fradiae and the features of this high-level DNA amplification are unique to Streptomyces among bacterial species.
Excision of chromosomal DNA sequences from Streptomyces coelicolor forms a novel family of plasmids detectable in Streptomyces lividans
TLDR
The autonomous SLP1 plasmids exist within S. lividans in a few copies per chromosome, and act as fertility factors, and provide suitable vectors for DNA cloning since the segments of chromosomal DNA carried by the larger members of the family are dispensable.
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TLDR
Recombination frequencies achieved by this technique are so high that selectable markers could be dispensed with, at least in certain kinds of strain improvement programmes, and the availability of a simple generally applicable procedure to recombine actinomycete strains at high frequency is reported.
Characterization of a plasmid from Streptomyces coelicolor A3(2)
Covalently closed circular deoxyribonucleic acid (DNA) with a molecular weight of 20 X 10(6) was identified in strains of Streptomyces coelicolor A3(2) of various fertility types. Hybridization
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TLDR
Recombination in the laboratory between unrelated species of organisms having little DNA sequence homology is ordinarily not feasible, however, it has long been apparent that great benefits could be derived from intergeneric, as well as intrageneric, genetic manipulations.
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TLDR
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TLDR
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