Transformation of plasmid DNA into Streptomyces at high frequency

  title={Transformation of plasmid DNA into Streptomyces at high frequency},
  author={Mervyn J. Bibb and Judith M. Ward and David. Hopwood},
OVER 60% of known antibiotics1 are produced by Streptomyces species, including many substances with valuable clinical and other applications; they therefore have considerable medical, biological and commercial importance. Although the process of gene exchange mediated by conjugation within these actinomycetes is apparently widespread2, the transfer of genetic material between individuals by such sexual means is, by definition, predominantly restricted to members of the same species. Transfer of… 
DNA cloning in Streptomyces: resistance genes from antibiotic-producing species
A method for molecular cloning involving the transfer of genes between unrelated streptomycetes is developed, which could facilitate the development of industrial strains with increased antibiotic yield, or capable of making new antibiotics4.
Conjugative sex plasmids of Streptomyces.
Much is known about the ways in which the conjugative plasmids of gram-negative bacteria cause their hosts to mate (1). Detailed knowledge has been obtained about how the transfer of plasmid DNA
Streptomyces cloning: useful recombinant DNA systems and a summation of cloned genes.
  • P. Tomich
  • Biology
    Antimicrobial Agents and Chemotherapy
  • 1988
An overview of the recombinant DNA systems available in Streptomyces spp.
Recent achievements in the generation of stable genome alterations/mutations in species of the genus Streptomyces
An attempt to provide a comprehensive summary of both approaches for stable genomic engineering and to outline recent advances in these strategies, such as CRISPR/Cas9, which have successfully manipulated Streptomyces strains to improve their biotechnological properties and increase production of natural or new gene-manipulated biologically active compounds.
Cloning of antibiotic resistance and nutritional genes in streptomycetes
Methodology which allows consistent shotgun cloning of streptomycete genes is presented. Parameters that increase transformation efficiency of Streptomyces lividans 66 were adjusted to generate
Models for genetic manipulation of Actinomycetes.
The ability to genetically manipulate these industrially important microorganisms should increase dramatically with the development of methodologies and techniques for DNA cloning in actinomycetes.
Plasmid loss and changes within the chromosomal DNA of Streptomyces reticuli
By using a new colony hybridization technique developed for streptomycetes, it could be shown that plasmidless variants could be transformed with the wild-type plasmids, which, however, is quickly lost from regenerated mycelium.
Uses of Recombinant DNA for Analyses of Streptomyces Species
Progress is described in the program to apply recombinant DNA technology to S. fradiae and the features of this high-level DNA amplification are unique to Streptomyces among bacterial species.
Excision of chromosomal DNA sequences from Streptomyces coelicolor forms a novel family of plasmids detectable in Streptomyces lividans
The autonomous SLP1 plasmids exist within S. lividans in a few copies per chromosome, and act as fertility factors, and provide suitable vectors for DNA cloning since the segments of chromosomal DNA carried by the larger members of the family are dispensable.


Genetic recombination through protoplast fusion in Streptomyces
Recombination frequencies achieved by this technique are so high that selectable markers could be dispensed with, at least in certain kinds of strain improvement programmes, and the availability of a simple generally applicable procedure to recombine actinomycete strains at high frequency is reported.
Characterization of a plasmid from Streptomyces coelicolor A3(2)
Covalently closed circular deoxyribonucleic acid (DNA) with a molecular weight of 20 X 10(6) was identified in strains of Streptomyces coelicolor A3(2) of various fertility types. Hybridization
DNA Cloning and the Analysis of Plasmid Structure and Function
Recombination in the laboratory between unrelated species of organisms having little DNA sequence homology is ordinarily not feasible, however, it has long been apparent that great benefits could be derived from intergeneric, as well as intrageneric, genetic manipulations.
Factors Affecting Infection of Protoplasts with Deoxyribonucleic Acid of Actinophage PK-66
To establish a method for transmission of genetic materials in the genus Streptomyces, the conditions of infection of protoplasts of S. kanamyceticus by actinophage PK-66 deoxyribonucleic acid (DNA)
Mechanism of bacterial transformation and transfection.
Characterization of Lethal Zygosis Associated with Conjugation in Escherichia coli K-12
The absence of lethal zygosis with filtrates and supernatant fluids from donors suggests a dependence on direct cell-cell contact as found in conjugation.
A site-specific endodeoxyribonuclease from Streptomyces albus CMI 52766 sharing site-specificity with Providencia stuartii endonuclease PstI.
A class II site-specific endodeoxyribonuclease (SalPI) was identified in cell-free extracts of Streptomyces albus CMI 52766 after high speed centrifugation and fractionation through Bio Gel AO.5M.
Isolation of covalently closed circular deoxyribonucleic acid from Streptomyces coelicolor A3(2)
Covalently closed circular deoxyribonucleic acid was isolated from two strains of Streptomyces coelicolor A3(2), representing two of the known fertility types, and the possible function of this DNA is discussed.