Transformation of peas (Pisum sativum L.) using immature cotyledons

  title={Transformation of peas (Pisum sativum L.) using immature cotyledons},
  author={Jan E. Grant and P. B. Armstrong. G. Beck. E. L. Cooper and Alastair E. McAra and Tonya J. Frew},
  journal={Plant Cell Reports},
A reliable Agrobacterium tumefaciens-mediated transformation method has been developed for peas (Pisum sativum) using immature cotyledons as the explant source. Transgenic plants were recovered from the four cultivars tested: Bolero, Trounce, Bohatyr and Huka. The method takes approximately 7 months from explant to seed-bearing primary regenerant. The binary vector used carried genes for kanamycin and phosphinothricin resistance. Transformed pea plants were selected on 10 mg/l phosphinothricin… CONTINUE READING


Publications citing this paper.
Showing 1-10 of 25 extracted citations


Publications referenced by this paper.

Grain Legumes 2:12-13

  • NJ Crampton
  • J Am Soc Hort Sei
  • 1975

Similar Papers

Loading similar papers…