Transformation of peanut (Arachis hypogaea L.) with tobacco chitinase gene: variable response of transformants to leaf spot disease.

@article{Rohini2001TransformationOP,
  title={Transformation of peanut (Arachis hypogaea L.) with tobacco chitinase gene: variable response of transformants to leaf spot disease.},
  author={V K. Rohini and K Sankara Rao},
  journal={Plant science : an international journal of experimental plant biology},
  year={2001},
  volume={160 5},
  pages={889-898}
}
Fertile transgenic plants of peanut (Arachis hypogaea L.) cv. TMV-2 expressing tobacco chitinase and neomycin phosphotransferase (npt II) genes were generated using an Agrobacterium tumefaciens (LBA4404/pBI121-pBTex)-mediated transformation system. A tissue culture-independent method wherein embryo in the mature seed is inoculated and reared into single plant transformant was used for transformation. Southern blot analysis of genomic DNA isolated from T(0) transformants and progeny plants (T(1… CONTINUE READING

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