Transformation of pea (Pisum sativum L.) byAgrobacterium tumefaciens

  title={Transformation of pea (Pisum sativum L.) byAgrobacterium tumefaciens},
  author={Johanna Puonti-Kaerlas and Priska Stabel and Tage Eriksson},
  journal={Plant Cell Reports},
Explants fromPisum sativum shoot cultures and epicotyls were transformed by cocultivation withAgrobacterium tumefaciens vectors carrying plant selectable markers and transformants could be selected on a medium containing kanamycin. Transformants could also be obtained at a low frequency by cocultivating small protoplast-derived colonies. The transformed nature of the calli obtained from selection was confirmed by opine assay and DNA analysis. In addition five cultivars of pea were tested for… CONTINUE READING
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