Transformation of lisianthus (Eustoma grandiflorum)

  title={Transformation of lisianthus (Eustoma grandiflorum)},
  author={Susan E Ledger and Simon C Deroles and David G. Manson and J. Marie Bradley and Nigel K. Given},
  journal={Plant Cell Reports},
Transformed plants from three cultivars of Eustoma grandiflorum (lisianthus) were produced by cocultivating young leaf pieces with Agrobacterium tumefaciens strain A722 containing the binary vectors pKIWI110 and pLN26. Both vectors contain the selectable marker gene for neomycin phosphotransferase II. pKIWI110 also contains the reporter gene for β-d-glucuronidase, and pLN26, the chalcone synthase antisense gene. Southern DNA analysis revealed that all the kanamycin-resistant transformants… CONTINUE READING
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