Transformation of eggplant (Solanum melongena L.) using a binary Agrobacterium tumefaciens vector

  title={Transformation of eggplant (Solanum melongena L.) using a binary Agrobacterium tumefaciens vector},
  author={Giuseppe Leonardo Rotino and Steve C Gleddie},
  journal={Plant Cell Reports},
Kanamycin resistant plants of Solarium melongena L. (eggplant) cv. Picentia were obtained following the cocultivation of leaf explants with Agrobacterium tumefaciens. A disarmed binary vector system containing the neomycin phosphotransferase (NPTII) gene as the selectable marker and chloramphenicol acetyltransferase (CAT) as a reporter gene was utilized. In vitro grown plants were used as sources of explants to produce transgenic plants on selective medium containing 100 mg/l kanamycin. The… CONTINUE READING
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Molecular c loning : a laboratory manual

  • J Niedermeyer, A Barnason, R Harsh, L FraleyR
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