Transformation of cultivated tomato by a binary vector in Agrobacterium rhizogenes: transgenic plants with normal phenotypes harbor binary vector T-DNA, but no Ri-plasmid T-DNA

@article{Shahin1986TransformationOC,
  title={Transformation of cultivated tomato by a binary vector in Agrobacterium rhizogenes: transgenic plants with normal phenotypes harbor binary vector T-DNA, but no Ri-plasmid T-DNA},
  author={Elias A. Shahin and Kitisri Sukhapinda and R. Bruce Simpson and Rosa Spivey},
  journal={Theoretical and Applied Genetics},
  year={1986},
  volume={72},
  pages={770-777}
}
Cultivated tomato was genetically transformed using two procedures. In the first procedure, punctured cotyledons were infected with “disarmed” Agrobacterium tumefaciens strain LBA4404 or with A. rhizogenes strain A4, each containing the binary vector pARC8. The chimeric neomycin phosphotransferase (NPT II) gene on pARC8 conferred on transformed plant cells the ability to grow on medium containing kanamycin. Transformation reproducible yielded kanamycin-resistant transformants in different… CONTINUE READING

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Rapid regeneration of plants from hypocotyl protoplasts and root segments of cabbage

  • C Lillo, EA Shahin
  • Hort Science
  • 1986
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