Transformation of chloroplasts with the psaB gene encoding a polypeptide of the photosystem I reaction center.

@article{Bingham1991TransformationOC,
  title={Transformation of chloroplasts with the psaB gene encoding a polypeptide of the photosystem I reaction center.},
  author={Scott E. Bingham and Rosa Xu and Andrew N. Webber},
  journal={FEBS letters},
  year={1991},
  volume={292 1-2},
  pages={137-40}
}
A chloroplast photosystem I reaction center mutation, ac-u-g-2.3, of Chlamydomonas reinhardtii has been complemented with a wild type psaB gene to restore photosynthetic competence. The mutation was mapped in the psaB coding sequence by chloroplast transformation using subcloned restriction fragments of psaB. The mutation was found to be a single base pair deletion resulting in a reading frame shift and premature termination of the polypeptide. Transformants were verified by insertion of a site… CONTINUE READING

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