Transformation of alfalfa chloroplasts and expression of green fluorescent protein in a forage crop

  title={Transformation of alfalfa chloroplasts and expression of green fluorescent protein in a forage crop},
  author={Zhengyi Wei and Yanzhi Liu and Chunjing Lin and Yunpeng Wang and Qin’an Cai and Yingshan Dong and Shaochen Xing},
  journal={Biotechnology Letters},
The ability to transform chloroplasts in multiple species is important for improving agricultural traits. Chloroplast transformation of alfalfa (Medicago sativa L.), a useful forage plant with high market value, was achieved using a vector carrying aadA and gfp genes being introduced into the chloroplasts of alfalfa via particle bombardment using leaves and calli as explants. Resistant somatic embryos were generated and developed into plantlets from explants. The transformation efficiency was 1… CONTINUE READING


Publications citing this paper.
Showing 1-10 of 11 extracted citations

Stable Expression of Basic Fibroblast Growth Factor in Chloroplasts of Tobacco

International journal of molecular sciences • 2015
View 3 Excerpts
Highly Influenced

Stable plastid transformation of rice, a monocot cereal crop.

Biochemical and biophysical research communications • 2018
View 1 Excerpt


Publications referenced by this paper.
Showing 1-10 of 35 references

Rapid screening method to detect potential biparental inheritance of plastid DNA and results for over 200 angiosperm species

JL Corriveau, AW Coleman
Amer J Bot • 1988
View 2 Excerpts
Highly Influenced

Alfalfa benefits from Medicago truncatula: the RCT1 gene from M. truncatula confers broad-spectrum resistance to anthracnose in alfalfa.

Proceedings of the National Academy of Sciences of the United States of America • 2008
View 1 Excerpt

Similar Papers

Loading similar papers…