Transformation of agrobacterium using the freeze-thaw method.

  title={Transformation of agrobacterium using the freeze-thaw method.},
  author={Detlef Weigel and Jane Glazebrook},
  journal={CSH protocols},
  volume={2006 7}
INTRODUCTIONThis protocol describes a method for transforming Agrobacterium with plasmid DNA using a freeze-thaw technique. Although the transformation efficiency for Agrobacterium is lower than that for Escherichia coli, it is possible to obtain adequate numbers of transformants with this technique.