Transformation of Trichoderma harzianum by high-voltage electric pulse

@article{Goldman2004TransformationOT,
  title={Transformation of Trichoderma harzianum by high-voltage electric pulse},
  author={Gustavo Henrique Goldman and Marc Van Montagu and Alfredo Herrera-Estrella},
  journal={Current Genetics},
  year={2004},
  volume={17},
  pages={169-174}
}
We have developed conditions for an efficient method of genetic transformation in Trichoderma harzianum, using high-voltage electroporation. Transformation was obtained with a plasmid carrying the Escherichia coli, hygromycin B phosphotransferase gene as a dominant selectable marker, and the gpd promoter and trpC terminator from Aspergillus nidulans. The… CONTINUE READING