Transformation of Schizosaccharomyces pombe: Protoplast Procedure.

Abstract

Transformation of Schizosaccharomyces pombe with DNA requires the conditioning of cells to promote DNA uptake followed by cell growth under conditions that select and maintain the plasmid or integration event. The three main methodologies are electroporation, treatment with lithium cations, and transformation of protoplasts. The protocol for protoplast… (More)
DOI: 10.1101/pdb.prot090977

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