Transformation of Rat Hepatocytes by Transfection with Simian Virus 40 DNA to Yield Proliferating Differentiated Cells1

Abstract

Cultured hepatocytes from adult Fischer 344 rats were transformed by virion or cloned simian virus 40 (SV40) DNA using the calcium phosphate method. Transformation by SV40 occurred in either serumsupplemented medium or chemically defined medium (COM). The fre quency was greatest in serum-supplemented medium but transformants did not remain differentiated… (More)

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