Transformation of Nonselectable Reporter Genes in Marine Diatoms

@article{Falciatore1999TransformationON,
  title={Transformation of Nonselectable Reporter Genes in Marine Diatoms},
  author={Angela Falciatore and Raffaella Casotti and Catherine Leblanc and Chiara Abrescia and Chris Bowler},
  journal={Marine Biotechnology},
  year={1999},
  volume={1},
  pages={239-251}
}
Abstract: We report the genetic transformation of two marine diatoms by microparticle bombardment. The pennate diatom Phaeodactylum tricornutum was transformed with the bacterial gene Sh ble from Streptoalloteichus hindustanus, which confers resistance to the antibiotics phleomycin and zeocin. Transformants contained between 1 and 10 copies of the exogenous DNA integrated into the genome by illegitimate recombination at apparently random locations. Transformation efficiencies were around 10−6… 
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