Transformation of Melon (Cucumis melo L.) and Expression from the Cauliflower Mosaic Virus 35S Promoter in Transgenic Melon Plants

@article{Dong1991TransformationOM,
  title={Transformation of Melon (Cucumis melo L.) and Expression from the Cauliflower Mosaic Virus 35S Promoter in Transgenic Melon Plants},
  author={Jin-zhuo Dong and M Yang and Shi-rong Jia and Nam-Hai Chua},
  journal={Bio/Technology},
  year={1991},
  volume={9},
  pages={858-863}
}
We have established a regeneration protocol for melon (Cucumis melo L.), using cotyledons as explants, in which the overall regeneration frequency reached more than 90 percent, and combined this protocol with Agrobacterium tumefaciens transformation to produce transgenic melon plants. Cotyledon explants were coculti-vated with A. tumefaciens carrying a disarmed Ti plasmid in which a dihydrofo-late reductase (DHFR) coding sequence and a β-glucuronidase (GUS) coding sequence were separately fused… CONTINUE READING

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