Transformation of Escherichia coli with large DNA molecules by electroporation.

  title={Transformation of Escherichia coli with large DNA molecules by electroporation.},
  author={Yan Sheng and Valeria Mancino and Bruce Birren},
  journal={Nucleic acids research},
  volume={23 11},
We have examined bacterial electroporation with a specific interest in the transformation of large DNA, i.e. molecules > 100 kb. We have used DNA from bacterial artificial chromosomes (BACs) ranging from 7 to 240 kb, as well as BAC ligation mixes containing a range o different sized molecules. The efficiency of electroporation with large DNA is strongly dependent on the strain of Escherichia coli used; strains which offer comparable efficiencies for 7 kb molecules differ in their uptake of 240… CONTINUE READING
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Molecular Cloning: A

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