Transformation of Diplonema papillatum, the type species of the highly diverse and abundant marine microeukaryotes Diplonemida (Euglenozoa)
@article{Kaur2018TransformationOD, title={Transformation of Diplonema papillatum, the type species of the highly diverse and abundant marine microeukaryotes Diplonemida (Euglenozoa)}, author={Binnypreet Kaur and Matus Valach and Priscila Pe{\~n}a-Diaz and Sandrine Moreira and P. Keeling and G. Burger and J. Luke{\vs} and Drahom{\'i}ra Faktorov{\'a}}, journal={Environmental Microbiology}, year={2018}, volume={20}, pages={1030–1040} }
Diplonema papillatum is the type species of diplonemids, which are among the most abundant and diverse heterotrophic microeukaryotes in the world's oceans. Diplonemids are also known for a unique form of post-transcriptional processing in mitochondria. However, the lack of reverse genetics methodologies in these protists has hampered elucidation of their cellular and molecular biology. Here we report a protocol for D. papillatum transformation. We have identified several antibiotics to which D… CONTINUE READING
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