Transformation of Candida maltosa and Pichia guilliermondii by a plasmid containing Saccharomyces cerevisiae ARG4 DNA

@article{Kunze1985TransformationOC,
  title={Transformation of Candida maltosa and Pichia guilliermondii by a plasmid containing Saccharomyces cerevisiae ARG4 DNA},
  author={Gotthard Kunze and C. Petzoldt and Ruediger Bode and Ida A. Samsonova and Michael Hecker and Daniel Birnbaum},
  journal={Current Genetics},
  year={1985},
  volume={9},
  pages={205-209}
}
Saccharomyces cerevisiae, Candida maltosa and Pichia guilliermondii have been transformed by the plasmid pYe(ARG4)411, which contains the S. cerevisiae ARG4 gene inserted into pBR322. In all transformants argininosuccinate lyase as well as β-lactamase were detected. The ARG+ phenotype of transformants is mitotically unstable. Closed circular pYe(ARG4)411, DNA was detected in transformant DNA preparations by hybridization to pBR322 DNA and by transformation of E. coli to ampicillin resistance.