Transformation of Arabidopsis thaliana with Agrobacterium tumefaciens

@article{Lloyd1986TransformationOA,
  title={Transformation of Arabidopsis thaliana with Agrobacterium tumefaciens},
  author={A. Lloyd and A. Barnason and S. G. Rogers and M. C. Byrne and R. Fraley and R. Horsch},
  journal={Science},
  year={1986},
  volume={234},
  pages={464 - 466}
}
  • A. Lloyd, A. Barnason, +3 authors R. Horsch
  • Published 1986
  • Biology, Medicine
  • Science
    • Transformed Arabidopsis thaliana plants have been produced by a modified leaf disk transformation-regeneration method. Leaf pieces from sterilely grown plants were precultured for 2 days and inoculated with an Agrobacterium tumefaciens strain containing an avirulent Ti (tumor-inducing) plasmid with a chimeric gene encoding hygromycin resistance. After cocultivation for 2 days, the leaf pieces were placed on a medium that selects for hygromycin resistance. Shoots regenerated within 3 months and… CONTINUE READING
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    References

    SHOWING 1-10 OF 22 REFERENCES
    A simple and general method for transferring genes into plants.
    • 4,134
    Improved protoplast culture and plant regeneration from protoplast-derived callus in Arabidopsis thaliana
    • 29
    Gene transfer in plants: Production of transformed plants using Ti plasmid vectors
    • 256
    In vitro Morphogenesis of Arabidopsis thaliana: The Origin of the Explant
    • 21
    Factors which Enhance in vitro Morphogenesis of Arabidopsis thaliana
    • 23
    Some Factors Controlling in vitro Morphogenesis of Arabidopsis thaliana
    • 27
    Callus proliferation and morphogenesis in tissue cultures of Arabidopsis thaliana L
    • 32
    Expression of prokaryotic genes for hygromycin B and G418 resistance as dominant-selection markers in mouse L cells.
    • 63
    T-DNA of the Agrobacterium TI and RI Plasmids
    • 153
    Arabidopsis thaliana and Plant Molecular Genetics
    • 252