Transformation of Arabidopsis thaliana with Agrobacterium tumefaciens

  title={Transformation of Arabidopsis thaliana with Agrobacterium tumefaciens},
  author={A. Lloyd and A. Barnason and S. G. Rogers and M. C. Byrne and R. Fraley and R. Horsch},
  pages={464 - 466}
  • A. Lloyd, A. Barnason, +3 authors R. Horsch
  • Published 1986
  • Biology, Medicine
  • Science
  • Transformed Arabidopsis thaliana plants have been produced by a modified leaf disk transformation-regeneration method. Leaf pieces from sterilely grown plants were precultured for 2 days and inoculated with an Agrobacterium tumefaciens strain containing an avirulent Ti (tumor-inducing) plasmid with a chimeric gene encoding hygromycin resistance. After cocultivation for 2 days, the leaf pieces were placed on a medium that selects for hygromycin resistance. Shoots regenerated within 3 months and… CONTINUE READING
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