Transformation of Arabidopsis thaliana with Agrobacterium tumefaciens

@article{Lloyd1986TransformationOA,
  title={Transformation of Arabidopsis thaliana with Agrobacterium tumefaciens},
  author={Alan Lloyd and Arlene Barnason and Stephen Gary Rogers and Michael C. Byrne and Robert T. Fraley and Robert B. Horsch},
  journal={Science},
  year={1986},
  volume={234},
  pages={464 - 466}
}
Transformed Arabidopsis thaliana plants have been produced by a modified leaf disk transformation-regeneration method. Leaf pieces from sterilely grown plants were precultured for 2 days and inoculated with an Agrobacterium tumefaciens strain containing an avirulent Ti (tumor-inducing) plasmid with a chimeric gene encoding hygromycin resistance. After cocultivation for 2 days, the leaf pieces were placed on a medium that selects for hygromycin resistance. Shoots regenerated within 3 months and… 
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This method for producing transformed plants combines gene transfer, plant regeneration, and effective selection for transformants into a single process and should be applicable to plant species that can be infected by Agrobacterium and regenerated from leaf explants.
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