Transformation of 2,4,6-Trinitrotoluene by Purified Xenobiotic Reductase B from Pseudomonas fluorescensI-C

  title={Transformation of 2,4,6-Trinitrotoluene by Purified Xenobiotic Reductase B from Pseudomonas fluorescensI-C},
  author={J. Pak and K. Knoke and D. Noguera and B. Fox and G. Chambliss},
  journal={Applied and Environmental Microbiology},
  pages={4742 - 4750}
  • J. Pak, K. Knoke, +2 authors G. Chambliss
  • Published 2000
  • Medicine, Chemistry
  • Applied and Environmental Microbiology
  • ABSTRACT The enzymatic transformation of 2,4,6-trinitrotoluene (TNT) by purified XenB, an NADPH-dependent flavoprotein oxidoreductase fromPseudomonas fluorescens I-C, was evaluated by using natural abundance and [U-14C]TNT preparations. XenB catalyzed the reduction of TNT either by hydride addition to the aromatic ring or by nitro group reduction, with the accumulation of various tautomers of the protonated dihydride-Meisenheimer complex of TNT, 2-hydroxylamino-4,6-dinitrotoluene, and 4… CONTINUE READING
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