Transferrin Receptors and Selective Iron Deposition in Pancreatic B Cells of Iron‐overloaded Rats

@article{Lu1991TransferrinRA,
  title={Transferrin Receptors and Selective Iron Deposition in Pancreatic B Cells of Iron‐overloaded Rats},
  author={J P Lu and K. Hayashi and Shoji Okada and Michiyasu Awai},
  journal={Pathology International},
  year={1991},
  volume={41}
}
Iron overload was produced in Wistar rats by repeated intraperitoneal injections of ferric nitrilotriacetate (Fe3+‐NTA) for one to six months. Pancreatic tissues from these iron‐overloaded rats and untreated controls were examined for insulin (for B cells), glucagon (for A cells), transferrin receptor (TfR), transferrin (Tf) and ferritin (Ft) using immunohistochemical methods, and for iron by histochemical Berlin blue staining. In the islets of iron‐overloaded rats, increased Ft staining… 
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References

SHOWING 1-10 OF 25 REFERENCES
Transferrin Receptor Expression in Normal, Iron‐deficient and Iron‐overloaded Rats
TLDR
It is concluded that TfR expression is negatively regulated by the tissue concentration of iron.
Induction of diabetes in animals by parenteral administration of ferric nitrilotriacetate. A model of experimental hemochromatosis.
TLDR
Repeated blood withdrawals from ferric-nitrilotriacetate-treated animals resulted in disappearance of hypergycmia, glycosuria, ketonemia, and ketonuria; disappearance of iron from the liver and pancreas; and restoration of islet beta granules to the control level.
Pancreatic Islets after Repeated Injection of Fe3+‐NTA An Ultrastructural Study of Diabetic Rats
TLDR
Repeated venesection therapy of rats injected with Fe3+‐NTA for 120 days resulted in an increase of morphologically normal B cells with a smaller number of necrotizing cells, accompanied by recovery from diabetic symptoms, and the toxic effect of injected iron on B cells was clarified.
Iron metabolism; the pathophysiology of iron storage.
TLDR
This sequence of events, whether following excessive iron absorption or parenteral iron administration is believed to be responsible for the clinical and pathologic picture of hemochromatosis.
Degranulation effect of ferric nitrilotriacetate (Fe3+-NTA) on the pancreatic islet beta-cells: its acute toxic effect on glucose metabolism.
TLDR
The results indicate a single Fe3+-NTA injection induced transitory instability of the pancreatic islet beta-cell granules and the glucose intolerance with a hyperresponse of IRI.
In vivo evidence for the functional heterogeneity of transferrin-bound iron. III. Studies of transferrin at high and low iron saturation.
TLDR
These studies add further support for the Fletcher-Huehns hypothesis of the functional heterogeneity of the transferrin iron pool in the rat.
ENDOCYTOSIS OF CATIONIC AND ANIONIC IRON COLLOID PARTICLES BY RAT MACROPHAGES
TLDR
The results indicate that the “non-specific receptor mediated phagocytosis” by macrophages is induced by the binding of anionic macromolecules to the cationic receptor on the cell surface.
Transferrin receptors and the uptake and release of iron by isolated hepatocytes
TLDR
Observations are consistent with a mechanism for iron uptake into hepatocytes involving the binding of iron‐transferrin to a specific cell‐surface receptor.
Iron release from haemosiderin and production of iron-catalysed hydroxyl radicals in vitro.
TLDR
Findings suggested that, under physiological conditions, haemosiderin was not an effective iron donor and was almost not involved in radical production, but under acidic conditions it could possibly be an Iron donor and is thought to be implicated inradical production and tissue damage in iron-overloaded conditions.
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