Transfer of the drug-resistance transposon Tn5 toErwinia herbicola and the induction of insertion mutations

@article{Gantotti2005TransferOT,
  title={Transfer of the drug-resistance transposon Tn5 toErwinia herbicola and the induction of insertion mutations},
  author={Basappa V. Gantotti and Karen L. Kindle and Steven V. Beer},
  journal={Current Microbiology},
  year={2005},
  volume={6},
  pages={377-381}
}
Translocatable drug-resistance element (transposon) Tn5 was transferred through conjugation from its carrier “suicidal” plasmid pJB4JI, harbored byEscherichia coli, toErwinia herbicola. The frequencies of transfer ranged from 0.4×10−8 to 26×10−8 per recipient cell. Membrane filter mating yielded more transconjugants than the spread plate technique. Several… CONTINUE READING