Transfer of sperm into a chemically defined environment by centrifugation through 12% (wt/vol) Accudenz.


Centrifugation is commonly used to wash sperm; however, most washing techniques do not put sperm in a chemically defined environment. Rather, washing by centrifugation, in effect, dilutes seminal plasma components. A 0.5-mL volume of 30% (wt/vol) Accudenz was layered beneath 5 mL of 12% (wt/vol) Accudenz in a 15-mL polypropylene centrifuge tube. Diluted… (More)

5 Figures and Tables


  • Presentations referencing similar topics