Transfer of methomyl and HmT-toxin sensitivity from T-cytoplasm maize to tobacco

Abstract

The mitochondrial gene, T-urf13, which is unique to the T-cytoplasm of maize, has been expressed in tobacco plants using the Cauliflower Mosaic Virus 35S promoter. Tobacco plants expressing T-urf13 exhibit a variety of responses to methomyl. Leaf discs and petiole sections bleach when exposed to methomyl or HmT-toxin; this effect increases with the age of the tissue. The bleaching effect is not however observed when light is excluded. Plants homozygous for T-urf13 exhibit extreme sensitivity when sprayed with methomyl. The growth of seedling which are either homozygous or heterozygous for T-urf13 is inhibited by methomyl and by kanamycin, whereas seedlings from untransformed tobacco or tobacco which has lost the T-urf13 gene through segregation are sensitive to kanamycin but develop normally when exposed to methomyl. The results demonstrate that T-URF13 need not be specifically targeted to the mitochondrion for it to induce methomyl or HmT-toxin sensitivity in tobacco.

DOI: 10.1007/BF00267463

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Cite this paper

@article{Allmen1991TransferOM, title={Transfer of methomyl and HmT-toxin sensitivity from T-cytoplasm maize to tobacco}, author={Jean-Marc von Allmen and William H. Rottmann and Burle G. Gengenbach and Alison J. Harvey and D. M. Lonsdale}, journal={Molecular and General Genetics MGG}, year={1991}, volume={229}, pages={405-412} }