Transfer of male or female primordial germ cells of quail into chick embryonic gonads.

@article{Ono1996TransferOM,
  title={Transfer of male or female primordial germ cells of quail into chick embryonic gonads.},
  author={Tamao Ono and Ryohei Yokoi and Hiroaki Aoyama},
  journal={Experimental animals},
  year={1996},
  volume={45 4},
  pages={
          347-52
        }
}
Blood from an individual quail embryo at stages 13-16, when primordial germ cells (PGCs) were in circulation, was taken from its marginal vein and transfused into the marginal vein of a chick embryo at stages 13-16. Both donor and recipient embryos were cultured in vitro until day 8 of development and their sex was determined by morphological and histological observations of the gonads. Sections of recipient gonads were stained immunohistochemically with QCR1 monoclonal antibody positive for… CONTINUE READING
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