Transfer of citrus tristeza virus (CTV)-derived resistance candidate sequences to four grapefruit cultivars through Agrobacterium-mediated genetic transformation

@article{Ananthakrishnan2007TransferOC,
  title={Transfer of citrus tristeza virus (CTV)-derived resistance candidate sequences to four grapefruit cultivars through Agrobacterium-mediated genetic transformation},
  author={Gopal Ananthakrishnan and Vladimir Orbovi{\'c} and Gemma Pasquali and M. {\'C}alovi{\'c} and Jude W. Grosser},
  journal={In Vitro Cellular & Developmental Biology - Plant},
  year={2007},
  volume={43},
  pages={593-601}
}
Transgenic plants of grapefruit (Citrus paradisi Macf.) cvs. ‘Duncan’, ‘Flame’, ‘Marsh’, and ‘Ruby Red’ were obtained using Agrobacterium tumefaciens-mediated transformation of seedling epicotyl tissue. Two citrus tristeza virus (CTV)-derived candidate resistance genes: ‘392’ (3′ region of the p23 ORF plus 3′ untranslated region—UTR) and ‘p23 hairpin’ (sense-p23 ORF plus UTR plus antisense-p23 ORF) were introduced into grapefruit using Agrobacterium strains EHA105 and EHA101, respectively… CONTINUE READING

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