Transfer of broad host-range plasmids to sulphate-reducing bacteria

@inproceedings{Powell1989TransferOB,
  title={Transfer of broad host-range plasmids to sulphate-reducing bacteria},
  author={Bridget J. Powell and Max Mergeay and Nicholas Christofi},
  year={1989}
}
The broad-host-range, IncQ, plasmid R300B (Sm, Su) has been stably transferred to two strains of sulphate-reducing bacteria (Desulfovibrio sp. 8301 and Desulfovibrio desulfuricans 8312), using the IncP1 transfer system of the helper plasmid pRK2013 and cocultivation of sulphate-reducing bacteria with facultative anaerobes in media provided with sulphate and nitrate ions as electron acceptors. R300B was transferred at a frequency of 10−2 to 1 per acceptor cell. The SmR marker was expressed in… CONTINUE READING

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