Transfer of a conjugative transposon, Tn5397 in a model oral biofilm.

@article{Roberts1999TransferOA,
  title={Transfer of a conjugative transposon, Tn5397 in a model oral biofilm.},
  author={Adam Paul Roberts and Jonathan Pratten and Michael Wilson and Peter Mullany},
  journal={FEMS microbiology letters},
  year={1999},
  volume={177 1},
  pages={63-6}
}
A tetracycline resistance profile was established from a microcosm dental plaque in a constant depth film fermenter. The fermenter was inoculated with a Bacillus subtilis strain which contained the conjugative transposon, Tn5397, which confers tetracycline resistance upon its host. After 6 hour and 24 hour the tetracycline resistance profile of the biofilm was redetermined and a tetracycline resistant Streptococcus species was isolated. A molecular analysis of this strain confirmed that Tn5397… CONTINUE READING