Transfection of plant mitochondria and in organello gene integration

  title={Transfection of plant mitochondria and in organello gene integration},
  author={Daria Mileshina and Milana V Koulintchenko and Yuri M Konstantinov and Andre Dietrich},
  booktitle={Nucleic acids research},
Investigation and manipulation of mitochondrial genetics in animal and plant cells remains restricted by the lack of an efficient in vivo transformation methodology. Mitochondrial transfection in whole cells and maintenance of the transfected DNA are main issues on this track. We showed earlier that isolated mitochondria from different organisms can import DNA. Exploiting this mechanism, we assessed the possibility to maintain exogenous DNA in plant organelles. Whereas homologous recombination… CONTINUE READING
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